Journal: Clinical and Translational Gastroenterology
Article Title: A Tissue-Based Comparative Effectiveness Analysis of Biomarkers for Early Detection of Colorectal Tumors
doi: 10.1038/ctg.2012.21
Figure Lengend Snippet: Promoter methylation status of the previously identified DNA methylation markers in normal and tumor tissue samples. The figure illustrates the methylation status of the previously identified DNA methylation biomarkers across test and validation sets of colorectal carcinomas ( n =169), adenomas ( n =104), normal mucosa samples from colorectal cancer (CRC)-free individuals ( n =107), and normal mucosa from CRC patients ( n =105). For comparison the methylation status of the novel epi-panel for early detection of CRC is included. Green color indicates unmethylated sample, whereas red color indicates methylated sample.
Article Snippet: In addition to patient samples, each plate included a standard curve generated from a 1:5 dilution series (32.5–0.052 ng) using a commercially available fully methylated positive DNA control (CpGenome Universal Methylated DNA; Millipore Billerica, MA, USA), multiple water blanks, positive controls (CpGenome Universal Methylated DNA), unmodified DNA, as well as bisulfite-treated normal blood as negative controls.
Techniques: Methylation, DNA Methylation Assay, Biomarker Discovery, Comparison